第２０回　生命医科学セミナー（Biomedical Science Seminar）

演題：Genomic Analysis of the Implantation Serine Proteinases

演者：Dr. Derrick E. Rancourt

所属：Faculty of Medicine, The University of Calgary, Canada

日時：平成１６年１０月２６日（火）　１７：３０～１８：３０

場所：コラボ・ステーションⅠ、１階共同セミナー室Ａ

［セミナー内容の紹介］

The S1 serine protease family is one of the largest gene families known. Within this family there are several subfamilies that have been grouped together as a result of sequence comparisons and substrate identification. The grouping of related genes allows for the speculation of function for newly found members by comparison and for novel subfamilies by contrast. Analysis of the evolutionary patterns of genes indicates whether or not orthologs are likely to be identified in other species as well as potentially indicating that hypothesized orthologs are in fact not. Looking at subtle differences between subfamily members can reveal intricacies about function and expression. Previously, we have described genes encoding two novel serine proteinases, ISP1 and ISP2, which are most closely related to tryptases. The ISP1 gene encodes the embryo-derived enzyme strypsin, which is necessary for blastocyst hatching and invasion in vitro. Additionally both ISP1 and ISP2 are co-expressed in the endometrial gland during the time of hatching, suggesting that they may also both participate in zona lysis from within the uterine lumen. Here, we demonstrate that the ISPs are tandemly linked within the tryptase cluster on 17A3.3. We suggest that remarkable similarities within the 50-untranslated and first intron regions of ISP1 and ISP2 may explain their intimate co-regulation in uterus. We also suggest that ISP genes have evolved through gene duplication and that the ISP1 gene has also begun to adopt an additional new function in the murine preimplantation embryo.

　本講演セミナーは、九州大学大学院医学研究院/医学系学府/医学部とカルガリー大学医学部との部局間交流の一環として開催致します。（共催：福岡医学会）