We will host a research seminar by Dr. R. Irene Jacobsen (from Moser lab in Norway). We welcome an audience from other campus!
Speaker: R. Irene Jacobsen
Kavli Institute for Systems Neuroscience and Centre for Neural Computation, NTNU, Trondheim, Norway
Title: A virus-based method for identifying inputs to a single neuron in mice
Date: Jan 12, 2021; Time: 17:00-18:00 JST
Venue: Zoom (Contact Takeshi Imai or Marcus N. Leiwe for the Zoom link)
Neuronal firing patterns are the result of a convergence of numerous inputs onto single cells. Elucidating these inputs, both anatomically and functionally, is essential in order to understand how neurons process information. Single-cell electroporation and expression of modified rabies viruses enables precise mapping of inputs to individual cells, but access to neurons in deep brain structures requires invasive procedures, including removal of overlying tissue.
We have developed a method that through a combination of virus injections allows us to target single cells with a G-protein deleted rabies virus and map their monosynaptic inputs without the use of electroporation or aspiration. We demonstrate the use of this method in the hippocampus, identifying both local and distant inputs to single neurons. By expanding the toolbox for monosynaptic input labelling, this method will help further our understanding of neuronal integration at the level of single cells
Host: Takeshi Imai, Graduate School of Medical Sciences, Kyushu University
#18th research seminar hosted by Imai lab