Department of Biochemistry

Kyushu University Faculty of Medical Sciences

Ikenouchi Lab

Publications

(† : Corresponding author)
(* : Co-first author)

  • CaMKII nucleates an osmotic protein supercomplex to induce cellular bleb expansion

    Fujii Y , Sakai Y , Matsuzawa K , †Ikenouchi J.

    EMBO J. 2026 Feb 3. Online ahead of print. [Pubmed]

  • Chemotherapy resistance due to epithelial-to-mesenchymal transition is caused by abnormal lipid metabolic balance

    *Matsumoto A , *Inoko A , Tanaka T, Konishi G, Hosoda W, Kojima T, Ohnishi K, †Ikenouchi J.

    Elife. 2026 Jan 12:13:RP104374. [Pubmed]

  • A steady-state pool of calcium-dependent actin is maintained by Homer and controls epithelial mechanosensation

    *Matsuzawa K , *Suzuki M , Cho Y , Fujinaga R , †Ikenouchi J.

    Proc Natl Acad Sci U S A. 2025 Oct 28;122(43):e2509784122. [Pubmed]

  • Rho-ROCK liberates sequestered claudin for rapid de novo tight junction formation

    Cho Y , Taniguchi A , Kubo A , †Ikenouchi J.

    Elife. 2025 Jul 24:13:RP102794. [Pubmed]

  • D-π-A Fluorophores with Strong Solvatochromism for Single-Molecule Ratiometric Thermometers

    Hori A, Matsumoto A, Ikenouchi J, †Konishi G.

    J Am Chem Soc. 2025 Mar 19;147(11):9953-9961. [Pubmed]

  • A sustained calcium response mediated by IP3 receptor anchoring to the desmosome is essential for apoptotic cell elimination

    Cho Y, Koyama-Honda I, Tanimura A, Matsuzawa K, † Ikenouchi J.

    Curr. Biol. 2024 Sep 17:S0960-9822(24)01173-4. [Pubmed]

  • Fluorescent Solvatochromic Probes for Long-Term Imaging of Lipid Order in Living Cells

    Tanaka T, Matsumoto A, A S Klymchenko, Tsurumaki E, † Ikenouchi J, † Konishi G.
    Adv Sci. 2024 Mar 11:e2309721. [Pubmed]

  • Cytoplasmic zoning in membrane blebs

    Fujii Y, †Ikenouchi J.

    J Biochem. 2024 Feb 25;175(2):133-140.  [Pubmed]

  • Accumulation of annexin A2 and S100A10 prevents apoptosis of apically delaminated, transformed epithelial cells

    Ito S, Kuromiya K, Sekai M, Sako H, Sai K, Morikawa R, Mukai Y, Ida Y, Anzai M, Ishikawa S, Kozawa K, Shirai T, Tanimura N, Sugie K, Ikenouchi J, Ogawa M, Naguro I, Ichijo H, †Fujita Y.

    Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2307118120. [Pubmed]

  • Cholesterol rich domain formation mediated by ZO proteins are essential for tight junction formation

    Shigetomi K, Ono Y, Matsuzawa K,†Ikenouchi J.

    Proc Natl Acad Sci U S A. 2023 Feb 21;120(8):e2217561120. [Pubmed]

  • mTORC2 suppresses cell death induced by hypo-osmotic stress by promoting sphingomyelin transport

    Ono Y, Matsuzawa K,† Ikenouchi J.

    J Cell Biol. 2022 Apr 4;221(4):e202106160. [Pubmed]

  • Tricellulin secures the epithelial barrier at tricellular junctions by interacting with actomyosin

    Cho Y, Haraguchi D, Shigetomi K, Matsuzawa K, Uchida S,† Ikenouchi J.

    J Cell Biol. 2022 Apr 4;221(4):e202009037. [Pubmed]

  • A Clockwork Bleb: cytoskeleton, calcium, and cytoplasmic fluidity

    †Ikenouchi J, Aoki K.

    FEBS J. 2021 Oct 6;16220.[Pubmed]

  • MAGIs regulate aPKC to enable balanced distribution of intercellular tension for epithelial sheet homeostasis

    Matsuzawa K, Ohga H, Shigetomi K, Shiiya T, Hirashima M,† Ikenouchi J.

    Commun. Biol. 2021 Mar 12;4(1):337.[Pubmed]

  • STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing

    Aoki K, Harada S, Kawaji K, Matsuzawa K, Uchida S, †Ikenouchi J.

    Nat Commun. 2021 Jan 20;12(1):480.[Pubmed]

  • Coordinated changes in cell membrane and cytoplasm during maturation of apoptotic bleb

    Aoki K, Satoi S, Harada S, Uchida S, Iwasa Y, †Ikenouchi J.

    Mol Biol Cell. 2020 Feb 12:mbcE19120691.[Pubmed]

  • Cell Adhesion Structures in Epithelial Cells Are Formed in Dynamic and Cooperative Ways

    Shigetomi K, †Ikenouchi J.

    Bioessays. 2019 Jul 4;1(7):e1800227.[Pubmed]

  • Roles of membrane lipids in the organization of epithelial cells: Old and new problems

    †Ikenouchi J.

    Tissue Barriers. 2018 Aug 29:1-8.[Pubmed]

  • α-Catenin Controls the Anisotropy of Force Distribution at Cell-Cell Junctions during Collective Cell Migration

    Matsuzawa K, Himoto T, Mochizuki Y, †Ikenouchi J.

    Cell Rep. 2018 Jun 19;23(12):3447-3456.[Pubmed]

  • Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation

    Tsuchiya M, Hara Y, Okuda M, Itoh K, Nishioka R, Shiomi A, Nagao K, Mori M, Mori Y, Ikenouchi J, Suzuki R, Tanaka M, Ohwada T, Aoki J, Kanagawa M, Toda T, Nagata Y, Matsuda R, Takayama Y, Tominaga M, †Umeda M.

    Nat Commun. 2018 May 24;9(1):2049.[Pubmed]

  • Adherens junctions influence tight junction formation via changes in membrane lipid composition

    Shigetomi K,  Ono Y, Inai T, †Ikenouchi J.

    J Cell Biol. 2018 May 2.doi:10.1083.[Pubmed]

  • Emphatic visualization of sphingomyelin-rich domains by inter-lipid FRET imaging using fluorescent sphingomyelins

    Kinoshita M, Ano H, Murata M, Shigetomi K, Ikenouchi J, †Matsumori N.

    Sci Rep. 2017 Dec 1;7(1):16801.[Pubmed]

  • Regulation of the epithelial barrier by post-translational modifications of tight junction membrane proteins

    Shigetomi K, †Ikenouchi J.

    J Biochem. 2017 Nov 24. doi: 10.1093/jb/mvx077[Pubmed]

  • DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility

    Nishimura T, Ito S, Saito H, Hiver S, Shigetomi K, Ikenouchi J, †Takeichi M.

    J Cell Biol. 2016 Nov 21;215(4):559-573.  [Pubmed]

  • How do cells sense actin cortex-free membrane?

    †Ikenouchi J.

    Cell Cycle. 2016 Oct 17;15(20):2687-8.  [Pubmed]

  • Membrane bleb : a seesaw game of two small GTPases

    †Ikenouchi J, Aoki K.

    mall GTPases. 2016 [Pubmed] (Review)

  • A RhoA and Rnd3 cycle regulates actin reassembly during membrane blebbing

    Aoki K., Maeda F, Nagasako T, Mochizuki Y, Uchida S, and †Ikenouchi J.

    Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):E1863-71.  [Pubmed]

  • CaMKII regulates the strength of the epithelial barrier

    Shiomi R, Shigetomi K, Inai T, Sakai M, †Ikenouchi J.

    Sci Rep. 2015 Aug 18;5:13262. [Pubmed]

  • Targeting cholesterol in a liquid-disordered environment by theonellamides modulates cell membrane order and cell shape

    Arita Y, Nishimura S, Ishitsuka R, Kishimoto T, Ikenouchi J, Ishii K, Umeda M, Matsunaga S, Kobayashi T, †Yoshida M.

    Chem Biol. 2015 May 21;22(5):604-10. [Pubmed]

  • EPLIN is a crucial regulator for extrusion of RasV12-transformed cells

    Ohoka A, Kajita M, Ikenouchi J, Yako Y, Kitamoto S, Kon S, Ikegawa M, Shimada T, Ishikawa S, †Fujita Y.

    J Cell Sci. 2015 Feb 15;128(4):781-9. [Pubmed]

  • Tricellulin regulates junctional tension of epithelial cells at tricellular contacts through Cdc42

    Oda Y, Otani T, Ikenouchi J, †Furuse M.
    J Cell Sci. 2014 Oct 1;127(Pt 19):4201-12. [Pubmed]

  • Sphingomyelin clustering is essential for the formation of microvilli

    †Ikenouchi J, Hirata M, Yonemura S, Umeda M.
    J Cell Sci. 2013 Aug 15;126(Pt 16):3585-92. [Pubmed]

  • Upregulated function of mitochondria-associated ER membranes in Alzheimer disease

    Area-Gomez E, Lara Castillo M, Tambini M, Guardia-Laguarta C, Ad J.C. de Groof, Madra M, Ikenouchi J, Umeda M, Bird T, Sturley S, and †Schon E.
    EMBO J. 2012 : 31(21):4106-23.  [Pubmed]

  • Lipid polarity is maintained in absence of tight junctions

    †Ikenouchi J, Suzuki M, Umeda K, Ikeda K, Taguchi R, Kobayashi T, Sato SB, Koyabashi T, Stolz DB, Umeda M
    J Biol Chem. 2012; 287(12):9525-33.  [Pubmed]

  • LSR defines cell corners for tricellular tight junction formation in epithelial cells

    Masuda S, Oda Y, Sasaki H, Ikenouchi J, Higashi T, Akashi M, Nishi E,† Furuse M.
    J Cell Sci. 2011; 124:548-55.  [Pubmed]

  • Phosphorylation state regulates the localization of Scribble at adherens junctions and its association with E-cadherin-catenin complexes

    Yoshihara K, Ikenouchi J, Izumi Y, Akashi M, Tsukita S,† Furuse M.
    Exp Cell Res. 2011; 317(4):413-22.  [Pubmed]

  • FRMD4A regulates epithelial polarity by connecting Arf6 activation with the PAR complex

    †Ikenouchi J, Umeda M.

    Proc Natl Acad Sci U S A. (Track Ⅱ) 2010; 107:748-53.  [Pubmed]

  • Loss of occludin affects tricellular localization of tricellulin

    †Ikenouchi J, Sasaki H, Tsukita S, Furuse M, Tsukita S.

    Mol Biol Cell. 2008; 19:4687-93.  [Pubmed]

  • Defining the roles of ｛beta｝-catenin/plakoglobin-null F9 cells

    Shimizu M, Fukunaga Y, Ikenouchi J, †Nagafuchi A.

    Mol Cell Biol. 2008; 28:825-35.  [Pubmed]

  • Requirement of ZO-1 for the formation of belt-like adherens junctions during epithelial cell polarization

    †Ikenouchi J, Umeda K, †Tsukita S, Furuse M, Tsukita S.

    J Cell Biol. 2007; 176:779-86.  [Pubmed]

  • ZO-1 and ZO-2 independentrly determine where claudins are polymerized in tight-junction strand formation

    * Umeda K,* Ikenouchi J, Katahira-Tayama S, Furuse K, Sasaki H, Nakayama M, Matsui T, †Tsukita S, Furuse M, Tsukita S.

    Cell 2006; 126:741-54.  [Pubmed]

  • Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells

    Ikenouchi J, Furuse M, Furuse K, Sasaki H, Tsukita S, †Tsukita S.

    J Cell Biol. 2005; 171:939-45.  [Pubmed]

  • Apical membrane and junctional complex formation during simple epithelial cell differentiation of F9 cells

    * Komiya S, * Shimizu M, * Ikenouchi J, Yonemura S, Matsui T, FUkunaga Y, Liu H, Endo F, Tsukita S, †Nagafuchi A.

    Genes Cells. 2005; 10:1065-80.  [Pubmed]

  • Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail

    Ikenouchi J, * Matsuda M, Furuse M, †Tsukita S.

    J Cell Sci. 2003; 116:1959-67.  [Pubmed]

  • Embryonic hydromyelia: cystic dilatation of the lumbosacral neural tube in human embryos

    Ikenouchi J, Uwabe C, Nakatsu T, Hirose M, †Shiota K.

    Acta Neuropathol. 2002; 103:248-54. [Pubmed]