2024\u5e744\u6708\u4ee5\u964d\uff08\u4e88\u5b9a\uff09\u306f\u652f\u63f4\u30bb\u30f3\u30bf\u30fc\u306b\u5229\u7528\u767b\u9332\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u307e\u305f\u3001ShareAID\u3092\u901a\u3057\u3066\u5229\u7528\u6599\u91d1\u306e\u652f\u6255\u3044\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\uff081\u6642\u9593\u3042\u305f\u308a1500\u5186\uff09\u3002<\/p>\n
\u6559\u80b2\u30fb\u7814\u7a76\u652f\u63f4\u30bb\u30f3\u30bf\u30fc<\/h3>\n
\u306e\u63b2\u8f09\u30da\u30fc\u30b8\u306f\u3053\u3061\u3089<\/a>\u3002\u5229\u7528\u30fb\u767b\u9332\u65b9\u6cd5\u306b\u3064\u3044\u3066\u306f\u3053\u3061\u3089\u3092\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002<\/p>\n \u306e\u30da\u30fc\u30b8\u306f\u3053\u3061\u3089<\/a>\u3002\u5229\u7528\u306b\u3042\u305f\u3063\u3066\u306f\u3001\u4eca\u4e95\u7814\u62c5\u5f53\u8005\u3092\u901a\u3057\u3066\u4e0b\u8a18\u306eGoogle Calendar\u3067\u4e88\u7d04\u3092\u884c\u3044\u3001\u7d19\u30d9\u30fc\u30b9\u306e\u4f7f\u7528\u7c3f\u3092\u4f5c\u6210\u3057\u307e\u3059\u304c\u3001\u540c\u6642\u306bShareAID\u3067\u3082\u4f7f\u7528\u7c3f\u3092\u4ed8\u3051\u3066\u4e0b\u3055\u3044\u3002ShareAID\u3067\u306e\u4f7f\u7528\u7c3f\u306b\u57fa\u3065\u304d\u3001\u6bce\u6708\u81ea\u52d5\u7684\u306b\u8ab2\u91d1\u3055\u308c\u307e\u3059\u3002<\/p>\n ShareAID\u306e\u767b\u9332\u30fb\u5229\u7528\u65b9\u6cd5\uff08\u7c21\u6613\u7248\uff09\u306f\u3053\u3061\u3089<\/a>\u3002<\/p>\n \u3053\u3061\u3089\u306e\u30e9\u30a4\u30c8\u30b7\u30fc\u30c8\u9855\u5fae\u93e1\uff08Miltenyi Biotec\u793eUltramicroscopy Blaze<\/a>\uff09\u306f\u3001\u81d3\u5668\u4e38\u3054\u3068\u306e3D\u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u304c\u53ef\u80fd\u306a\u9855\u5fae\u93e1\u3067\u3059\u3002\u30b5\u30a4\u30ba\u306e\u5927\u304d\u306a\u900f\u660e\u5316\u6a19\u672c\u306e\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306b\u7279\u5316\u3057\u305f\u6a5f\u7a2e\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002 Objective lens:<\/strong><\/p>\n 1.1x (NA 0.1, WD 17 mm) Dipping cap:<\/strong><\/p>\n \u5c48\u6298\u7387\u306b\u3088\u3063\u3066\u4f7f\u3044\u5206\u3051\u3092\u884c\u3044\u307e\u3059\u3002\u5c48\u6298\u7387\u306b\u95a2\u3057\u3066\u306f\u6c34\uff081.33\uff09\u304b\u3089\u6709\u6a5f\u6eb6\u5a92\uff081.57\uff09\u307e\u3067\u30ab\u30d0\u30fc\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u57fa\u672c\u7684\u306b\u307b\u3068\u3093\u3069\u306e\u900f\u660e\u5316\u6cd5\u306b\u5bfe\u5fdc\u53ef\u80fd\u3067\u3059\u3002<\/p>\n water (RI 1.33-1.41) Laser:<\/strong><\/p>\n 445, 488, 515, 561, 639 nm<\/p>\n \u9752\u8272\uff08405\uff09\u30ec\u30fc\u30b6\u30fc\u306f\u900f\u904e\u7387\u304c\u60aa\u3044\u305f\u3081\u642d\u8f09\u3057\u3066\u3044\u307e\u305b\u3093\u3002\u3053\u306e\u305f\u3081\u3001DAPI\u3084BFP\u306f\u4f7f\u3048\u307e\u305b\u3093\u3002\u6838\u67d3\u8272\u304c\u5fc5\u8981\u306a\u5834\u5408\u306fRedDot2\uff08639nm\u52b1\u8d77\uff09\u3092\u7528\u3044\u307e\u3059\u304c\u3001\u5168\u8133registration\u306b\u95a2\u3057\u3066\u306f\u81ea\u5bb6\u86cd\u5149\u3092\u4f7f\u3048\u3070\u5341\u5206\u306a\u3053\u3068\u304c\u307b\u3068\u3093\u3069\u3067\u3059\u3002<\/p>\n Emission filter (\u4e2d\u5fc3\u3001\u5e45; nm):<\/strong><\/p>\n 480\/40 (CFP) Camera:<\/strong><\/p>\n 2560 x 2160, QY max 60%<\/p>\n PC:<\/strong><\/p>\n RAM 128 GB, HDD: 10TB x 1, 16 TB x 2, NVIDIA Quadro P1000(4GB)<\/p>\n \u30b5\u30f3\u30d7\u30eb\u99c6\u52d5\u57df\uff08X,Y,Z)\uff1a<\/strong><\/p>\n 24mm,50mm,23mm<\/p>\n \u5149\u30b7\u30fc\u30c8\u539a\uff1a<\/strong><\/p>\n 4-24 um<\/p>\n \u4e88\u7d04\u8868\u306f\u3053\u3061\u3089\uff08Google Calendar\uff09<\/a><\/p>\n \u3053\u3061\u3089\u306e\u30ab\u30ec\u30f3\u30c0\u30fc\u306f\u95b2\u89a7\u306e\u307f\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u4e88\u7d04\u306f\u4eca\u4e95\u7814\u306e\u62c5\u5f53\u8005\u3092\u901a\u3057\u3066\u884c\u3044\u307e\u3059\u3002\u5229\u7528\u5f8c\u306fShareAID\u306e\u30da\u30fc\u30b8<\/a>\u3092\u901a\u3057\u3066\u5229\u7528\u7c3f\u3092\u4ed8\u3051\u3066\u4e0b\u3055\u3044\u3002<\/p>\n \u30c7\u30fc\u30bf\u30b5\u30a4\u30ba\u304c\u81a8\u5927\u3068\u306a\u308b\u305f\u3081\u3001\u3068\u308a\u3042\u3048\u305a\u9ad8\u89e3\u50cf\u5ea6\u3067\u81d3\u5668\u4e38\u3054\u3068\u64ae\u50cf\u3059\u308b\u3001\u3068\u3044\u3046\u3053\u3068\u306f\u30aa\u30b9\u30b9\u30e1\u3057\u307e\u305b\u3093\u3002\u9ad8\u89e3\u50cf\u5ea6\u3067\u898b\u305f\u3051\u308c\u3070\u5171\u7126\u70b9\u9855\u5fae\u93e1\u306e\u65b9\u304c\u826f\u3044\u3067\u3059\u3002\u5b9f\u9a13\u306e\u76ee\u7684\u306b\u5408\u308f\u305b\u3066\u5b9f\u9a13\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3053\u3068\u304c\u5fc5\u9808\u3067\u3059\u3002\u81d3\u5668\u4e38\u3054\u3068\u306e\u30c7\u30fc\u30bf\u306b\u306a\u308b\u3068\u3001\u3054\u81ea\u5206\u306ePC\u3067\u306f\u753b\u50cf\u304c\u958b\u3051\u306a\u3044\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3067\u3059\u3002<\/p>\n \u30de\u30a6\u30b9\u5168\u8133\u3092\u3068\u308a\u3042\u3048\u305a\u64ae\u50cf\u3059\u308b\u5834\u5408\u306f1.1x\u30ec\u30f3\u30ba\u3067\u64ae\u50cf\u3057\u307e\u3059\uff08\u53f3\u306e\u5199\u771f\uff1a\u3053\u306e\u5834\u5408\u306f\u753b\u50cf\u306estitching\u4e0d\u8981\u3001\u6240\u8981\u6642\u9593\u306f1\u6642\u9593\/\u30c1\u30e3\u30f3\u30cd\u30eb\u4ee5\u5185\uff09\u3002\u81ea\u52d5\u3067\u30ec\u30b8\u30b9\u30c8\u30ec\u30fc\u30b7\u30e7\u30f3\u3068\u5b9a\u91cf\u3092\u884c\u3044\u305f\u3044\u5834\u5408\u306f4x\u30ec\u30f3\u30ba\u3067\u64ae\u50cf\uff08\u6240\u8981\u6642\u9593\u306f\u534a\u65e5\u7a0b\u5ea6\/\u30c1\u30e3\u30f3\u30cd\u30eb\uff09\u3057\u3001stitching\u3057\u307e\u3059\u3002<\/p>\n \u795e\u7d4c\u7a81\u8d77\u307e\u3067\u89b3\u5bdf\u3057\u305f\u3044\u5834\u5408\u306f12x\u306e\u30ec\u30f3\u30ba\u3092\u4f7f\u7528\u3002\u4f46\u3057\u300112x\u3067\u5168\u8133\u753b\u50cf\u3092\u53d6\u5f97\u3059\u308b\u3053\u3068\u306f\u307e\u305a\u4e0d\u53ef\u80fd\u306a\u306e\u3067\u30011.1x\u306a\u3044\u30574x\u306e\u753b\u50cf\u306b\u57fa\u3065\u3044\u3066\u3001\u898b\u305f\u3044\u5834\u6240\u3060\u3051\u3092\u64ae\u50cf\u3059\u308b\u3053\u3068\u304c\u73fe\u5b9f\u7684\u3067\u3059\u3002<\/p>\n \u30de\u30a6\u30b9\u306e\u5168\u8133\u3092\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3059\u308b\u5834\u5408\u306e\u30c7\u30fc\u30bf\u30b5\u30a4\u30ba\u306e\u76ee\u5b89\u3068\u3057\u3066\u306f\u30011.1x\u30ec\u30f3\u30ba\u3067\u3042\u308c\u3070\u657010GB\/\u30c1\u30e3\u30f3\u30cd\u30eb\u30014x\u30ec\u30f3\u30ba\u3067\u3042\u308c\u3070\uff5e500GB\/\u30c1\u30e3\u30f3\u30cd\u30eb\u300212x\u30ec\u30f3\u30ba\u3067\u5168\u8133\u64ae\u50cf\u3059\u308b\u3053\u3068\u306f\u73fe\u5b9f\u7684\u306b\u4e0d\u53ef\u80fd\u3002\u30c7\u30fc\u30bf\u89e3\u6790\u306b\u3042\u305f\u3063\u3066\u306f\u3001\u30c7\u30fc\u30bf\u30b5\u30a4\u30ba\u00d73\u500d\u306e\u30e1\u30e2\u30ea\uff08RAM\uff09\u304c\u5fc5\u8981\u306a\u305f\u3081\u3001\u30cf\u30a4\u30b9\u30da\u30c3\u30af\u306a\u5c02\u7528\u306e\u89e3\u6790\u30de\u30b7\u30f3\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n \u5f53\u9762\u306fCUBIC-L\/R\uff08\u5c48\u6298\u73871.52\uff09\u3067\u904b\u7528\u3059\u308b\u4e88\u5b9a\u3067\u3059\u3002\u5e0c\u671b\u304c\u3042\u308c\u3070\u3001SeeDB2G\uff08CLARITY\uff09\u3084BABB\u3082\u5bfe\u5fdc\u53ef\u80fd\u3067\u3059\u304c\u3001\u5bfe\u7269\u30ec\u30f3\u30ba\u306edipping cap\u4ea4\u63db\u3068\u5149\u8ef8\u8abf\u6574\u3001\u64ae\u50cf\u7528\u30aa\u30a4\u30eb\u306e\u4ea4\u63db\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n \u3068\u308a\u3042\u3048\u305a\u81d3\u5668\u3092\u4e38\u3054\u3068\u898b\u305f\u3044\u5834\u5408\u3001\u307e\u305a\u306fCUBIC-L\/R\u3092\u4f7f\u7528\u3002CUBIC-L\u306f\u81ea\u4f5c\u53ef\u80fd\u3060\u304c\u3001CUBIC-R+ (M)\u306f\u5e02\u8ca9\u54c1\uff08TCI, #T3741<\/a>\uff09\u3092\u4f7f\u7528\u3002\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8f1d\u5ea6\u3092\u6700\u5927\u9650\u4fdd\u6301\u3057\u305f\u3044\u5834\u5408\u306f\u3001Scale-CUBIC1 (or CUBIC-L)\u3067\u8131\u8102\u5f8c\u3001SeeDB2G\u3067\u900f\u660e\u5316\u3059\u308b\u3068\u826f\u3044\u3002\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u892a\u8272\u3057\u3066\u3082\u554f\u984c\u7121\u304f\u3001CUBIC\u3067\u306f\u900f\u660e\u5ea6\u304c\u4e0d\u5341\u5206\u306a\u5834\u5408\u306f\u30013DISCO\u3001BABB\u3001ECi\u306a\u3069\u3001\u6709\u6a5f\u6eb6\u5a92\u7cfb\u306e\u900f\u660e\u5316\u6db2\u3092\u691c\u8a0e\u3002\u7279\u306b\u30d2\u30c8\u6a19\u672c\u306f\u6709\u6a5f\u6eb6\u5a92\u7cfb\u3067\u306e\u5b9f\u7e3e\u304c\u591a\u3044\u3002<\/p>\n \u30c7\u30fc\u30bf\u8ee2\u9001\u306b\u6642\u9593\u304c\u639b\u304b\u308b\u305f\u3081\u3001\u30c7\u30fc\u30bf\u3092\u6301\u3061\u51fa\u3059\u969b\u306e\u5916\u90e8\u8a18\u61b6\u5a92\u4f53\u306f\u9ad8\u901fSSD\uff08PCIe 3.0 or 4.0\uff09\u306e\u307f\u3068\u3057\u307e\u3059\u30024TB\u7a0b\u5ea6\u304c\u6bd4\u8f03\u7684\u5b89\u4fa1\u3067\u4f7f\u3044\u3084\u3059\u3044\u3002\u5916\u4ed8\u3051HDD\u306f\u975e\u63a8\u5968\u3002<\/p>\n CUBIC-L\/R\u306b\u3088\u308b\u30de\u30a6\u30b9\u5168\u8133\u900f\u660e\u5316\u30d7\u30ed\u30c8\u30b3\u30eb<\/strong><\/p>\n Sample preparation<\/span><\/p>\n \u3057\u3070\u3057\u3070\u3001\u304a\u6301\u3061\u9802\u3044\u305f\u5168\u8133\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u7528\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u81ea\u5bb6\u86cd\u5149\u304c\u9ad8\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u306f\u704c\u6d41\u304c\u4e0d\u5341\u5206\u3067\u8840\u6db2\u304c\u6b8b\u3063\u3066\u3044\u308b\u305f\u3081\u3060\u3068\u601d\u308f\u308c\u307e\u3059\u30024% PFA\u3067\u6f45\u6d41\u56fa\u5b9a\u3059\u308b\u524d\u306b\u3001PBS 10 mL\u7a0b\u5ea6\u3067\u704c\u6d41\u3057\u3001\u5341\u5206\u306b\u8131\u8840\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002<\/p>\n Delipidation with CUBIC-L<\/span><\/p>\n 1. Take the sample and immerse it in 4% PFA at 4\u2103 for 24hr (with gentle shake)<\/p>\n 2. Wash the sample with PBS, 1hr \u00d73 times at RT (with gentle shake)<\/p>\n 3. Immerse the sample in CUBIC-L (30-40 mL in 50 mL tube) at 37\u2103 for 6 days (with shake)<\/p>\n (Replace the CUBIC-L with a new tube on Day 2 or 3 )<\/p>\n It’s not needed to exchange CUBIC-L every day. Shaking speed is important (e.g. reciprocating shaker for 50~100 rpm).<\/p>\n 4. Wash the sample with PBS, RT, 2hr\u00d73 times (with gentle shake)<\/p>\n Counterstaining<\/span><\/p>\n 5. Dilute the Propidium Iodide (ex 532) or RedDot2 (ex 637) with the staining buffer<\/p>\n 6. Immerse the sample in the antibody diluted staining buffer, RT for 4-5 days<\/p>\n 7. Wash the sample with PBS 1 hr \u00d73 RT (with gentle shake)<\/p>\n Final Clearing for index matching<\/span><\/p>\n 8. Immerse the sample in 10mL of 1\/2-diluted CUBIC-R (diluted with miliQ) at RT for 5-10 h with shake (we recommend 50 mL tube)<\/p>\n 9. Immerse the sample in 10mL of CUBIC-R at RT for 10h or overnight with shake (in 50 mL tube)<\/p>\n 10. Exchange the CUBIC-R and shake overnight x3 times at RT (in 50 mL tube)<\/p>\n \u6ce8\u610f\u70b9\uff1a<\/strong><\/p>\n 10mL\u306eCUBIC-R\u3067RI matching\u3092\u884c\u3046\u5834\u5408\u3001\u6700\u4f4e\u3067\u30823\u56de\u6eb6\u6db2\u3092\u4ea4\u63db<\/strong>\u3057\u3066overnight\u51e6\u7406\u3092\u7e70\u308a\u8fd4\u3059\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u3092\u5c48\u6298\u7387\u304c1.52\u306e\u30aa\u30a4\u30eb\u306b\u6c88\u3081\u3066\u64ae\u50cf\u3057\u307e\u3059\u306e\u3067\u3001\u900f\u660e\u5316\u30b5\u30f3\u30d7\u30eb\u306e\u5c48\u6298\u7387\u304c\u5b8c\u5168\u306b1.52\u306b\u306a\u3089\u306a\u3044\u3068\u304d\u308c\u3044\u306a\u50cf\u304c\u5f97\u3089\u308c\u307e\u305b\u3093\u3002<\/p>\n CUBIC-L<\/span><\/p>\n 1. Triton-X 50mL<\/p>\n 2. N-Butyldiethanolamine (TCI, B0725) 50mL<\/p>\n 3. dH2O 400mL<\/p>\n CUBIC-R+ (M)<\/span><\/p>\n TCI (T3741)<\/p>\n Staining buffer<\/span><\/p>\n 500 mM NaCl-PBS<\/p>\n \u30de\u30a6\u30b9\u5168\u8133\u6297\u4f53\u67d3\u8272<\/strong><\/p>\n \u6211\u3005\u306e\u65b9\u3067\u306f\u3042\u307e\u308a\u7d4c\u9a13\u304c\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u6297\u4f53\u67d3\u8272\u3092\u3059\u308b\u5834\u5408\u306f\u6709\u6a5f\u6eb6\u5a92\u7cfb\u306e\u900f\u660e\u5316\u6db2\u306e\u65b9\u304c\u826f\u3044\u3067\u3059\u3002\u30df\u30eb\u30c6\u30cb\u30fc\u793e\u306e\u3053\u3061\u3089\u306e\u8cc7\u6599<\/a>\u3092\u53c2\u7167\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n \u9855\u5fae\u93e1\u672c\u4f53\u3067\u306f\u753b\u50cf\u53d6\u5f97\u3057\u304b\u884c\u3048\u307e\u305b\u3093\u3002\u5927\u91cf\u306e\u753b\u50cf\u306e\u8cbc\u308a\u5408\u308f\u305b\u3084\u5b9a\u91cf\u306a\u3069\u3001\u89e3\u6790\u306f\u5404\u81ea\u3067\u884c\u3046\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u57fa\u672c\u7684\u306b\u306fImageJ\/Fiji<\/a>\u3084Imaris<\/a>\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002Imaris\/Fiji\u306f\u30d5\u30ea\u30fc\u30bd\u30d5\u30c8\u3067\u3059\u304c\u3001Imaris\u306f\u6709\u6599\u30bd\u30d5\u30c8\u306e\u305f\u3081\u3001\u5171\u901a\u6a5f\u5668\u7b49\u3092\u4f7f\u3046\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u53ef\u8996\u5316\u306e\u307f\u53ef\u80fd\u306aImaris Viewer<\/a>\u306f\u30d5\u30ea\u30fc\u3067\u3059\u304c\u3001\u5b9a\u91cf\u3084\u52d5\u753b\u4f5c\u6210\u3092\u3059\u308b\u5834\u5408\u306f\u6709\u6599\u7248\u304c\u5fc5\u8981\u3067\u3059\u3002\u5f53\u7814\u7a76\u5ba4\u3067\u884c\u3063\u3066\u3044\u308b\u30de\u30a6\u30b9\u5168\u8133\u89e3\u6790\u306e\u6d41\u308c\u306f\u4ee5\u4e0b\u306e\u901a\u308a\u3002<\/p>\n \u53d6\u5f97\u3057\u305f\u753b\u50cf\u306f\u3001\u5fc5\u8981\u306b\u5fdc\u3058\u3066ImageJ\/Fiji\u306eBigStitcher<\/a>\u3067\u753b\u50cf\u306estitching\u3092\u884c\u3046\u3002\u753b\u50cf\u306e\u53ef\u8996\u5316\u306fImaris Viewer<\/a>\u3092\u4f7f\u7528\u53ef\u80fd\u3002binning\u3068coronal\u753b\u50cf\u3078\u306e\u5909\u63db\u3092\u884c\u3063\u305f\u5f8c\u3001BIRDS<\/a>\u3067\u6a19\u6e96\u8133CCFv3\u3078\u306e\u81ea\u52d5registration\u3092\u884c\u3046\u3002\u89e3\u6790PC\u306e\u30e1\u30e2\u30ea\u306f\u753b\u50cf\u30b5\u30a4\u30ba\u306e3\u500d\u4ee5\u4e0a\u306f\u5fc5\u8981\uff08\u6700\u4f4e128GB\uff09\u306a\u306e\u3067\u6ce8\u610f\u3002<\/p>\n \u5171\u901a\u6a5f\u5668\u5ba4\u306b\u3082Imaris\u304c\u3042\u308b\u304c\u3001PC\u306e\u30e1\u30e2\u30ea\u304c128GB\u306e\u305f\u3081\u6271\u3048\u308b\u30c7\u30fc\u30bf\u30b5\u30a4\u30ba\u306b\u5236\u9650\u304c\u3042\u308b\uff08binning\u306b\u3088\u3063\u3066\u30c7\u30fc\u30bf\u30b5\u30a4\u30ba\u3092\u6e1b\u3089\u3055\u306a\u3044\u3068\u753b\u50cf\u30d5\u30a1\u30a4\u30eb\u3092\u958b\u3051\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308b\uff09\u3002\u4eca\u4e95\u7814\u306eImaris\u30de\u30b7\u30f3\u306f1.5TB\u306e\u30e1\u30e2\u30ea\u3092\u642d\u8f09\u3057\u3066\u3044\u308b\u306e\u3067\u5927\u5bb9\u91cf\u30c7\u30fc\u30bf\u306e\u89e3\u6790\u3082\u53ef\u80fd\u3002<\/p>\n \u4ed6\u306e\u81d3\u5668\u306b\u95a2\u3057\u3066\u306f\u30ce\u30a6\u30cf\u30a6\u304c\u9650\u3089\u308c\u3066\u3044\u308b\u306e\u3067\u5fdc\u76f8\u8ac7\u3068\u306a\u308a\u307e\u3059\u3002\u6a5f\u68b0\u5b66\u7fd2\u3092\u4f7f\u3063\u3066\u5bfe\u8c61\u7269\u3092\u81ea\u52d5\u691c\u51fa\u3059\u308b\u969b\u306b\u306filastik<\/a>\u306a\u3069\u3082\u4f7f\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n \u3053\u3061\u3089\u306e\u6a5f\u5668\u306f\u5b66\u5185\u5171\u901a\u6a5f\u5668\uff08\u6559\u80b2\u30fb\u7814\u7a76\u652f\u63f4\u30bb\u30f3\u30bf\u30fc<\/a>\u7ba1\u7406\uff09\u3068\u306a\u3063\u3066\u304a\u308a\u307e\u3059\u306e\u3067\u3001\u6210\u679c\u767a\u8868\u306e\u969b\u306b\u306f\u3053\u3061\u3089\u306e\u8a18\u8f09\u4f8b<\/a>\u306b\u5f93\u3063\u3066\u8b1d\u8f9e\u3092\u304a\u9858\u3044\u3057\u307e\u3059\u3002<\/p>\n \u30b5\u30dd\u30fc\u30c8\u62c5\u5f53\u8005\u306b\u5bfe\u3059\u308b\u8b1d\u8f9e\u4f8b\uff1aWe thank xxx for technical help on light-sheet microscopy.<\/p>\n \u5927\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u89e3\u6790\u3084\u3001\u65b0\u3057\u3044\u30c7\u30fc\u30bf\u89e3\u6790\u3092\u5fc5\u8981\u3068\u3059\u308b\u30b5\u30dd\u30fc\u30c8\u306a\u3069\u3001\u5171\u540c\u7814\u7a76\u30d9\u30fc\u30b9\u3067\u9032\u3081\u308b\u5834\u5408\u306f\u5225\u9014\u4eca\u4e95\u307e\u3067\u3054\u76f8\u8ac7\u4e0b\u3055\u3044\u3002<\/p>\n \u73fe\u5728\u3001\u30c6\u30b9\u30c8\u30e9\u30f3\u4e2d\u3067\u3059\u3002\u753b\u50cf\u53d6\u5f97\u3084\u89e3\u6790\u306e\u30b5\u30dd\u30fc\u30c8\u3001\u5171\u540c\u7814\u7a76\u306e\u3054\u76f8\u8ac7\u306f\u4eca\u4e95\u307e\u3067\u3002\u5b66\u5916\u306b\u3064\u3044\u3066\u306f\u5171\u540c\u7814\u7a76\u30d9\u30fc\u30b9\u3067\u306e\u307f\u306e\u53d7\u3051\u5165\u308c\u307e\u3059\u3002\u5b66\u8853\u5909\u9769\u9818\u57df\u306e\u65b9\u306f\u3053\u3061\u3089\uff08gakuhen.dynamic.brain@gmail.com\uff09\u307e\u3067\u3054\u76f8\u8ac7\u4e0b\u3055\u3044\u3002\u30e1\u30fc\u30eb\u306e\u3084\u308a\u3068\u308a\u306fPI\u304c\u76f4\u63a5\u3001\u3082\u3057\u304f\u306fPI\u3092CC\u306b\u5165\u308c\u305f\u4e0a\u3067\u304a\u9858\u3044\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":" \u3054\u5229\u7528\u306e\u6d41\u308c \u3053\u3061\u3089\u306e\u30e9\u30a4\u30c8\u30b7\u30fc\u30c8\u9855\u5fae\u93e1\u306f\u4e5d\u5927\u306e\u7814\u7a76\u74b0\u5883\u6574\u5099\u4e8b\u696d\u306b\u3088\u308a\u5c0e\u5165\u3055\u308c\u305f\u3082\u306e\u3067\u3001\u5b66\u5185\u5171\u901a\u6a5f\u5668\uff08\u6559\u80b2\u30fb\u7814\u7a76\u652f\u63f4\u30bb\u30f3\u30bf\u30fc\u7ba1\u7406\uff09\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u304c\u3001\u9855\u5fae\u93e1\u306e\u4f7f\u7528\u304a\u3088\u3073\u89e3\u6790\u306e\u5c02\u9580\u6027\u304c\u9ad8\u3044\u3053\u3068\u304b\u3089\u3001\u5f53\u9762\u306e\u9593\u3001\u4eca\u4e95\u7814\u306b\u3066\u5229\u7528\u306e… <\/p>\n","protected":false},"author":22,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2204","page","type-page","status-publish","hentry","clearfix"],"_links":{"self":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-json\/wp\/v2\/pages\/2204","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-json\/wp\/v2\/users\/22"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-json\/wp\/v2\/comments?post=2204"}],"version-history":[{"count":53,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-json\/wp\/v2\/pages\/2204\/revisions"}],"predecessor-version":[{"id":2744,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-json\/wp\/v2\/pages\/2204\/revisions\/2744"}],"wp:attachment":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-json\/wp\/v2\/media?parent=2204"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}ShareAID<\/h3>\n
\u9855\u5fae\u93e1\u306e\u4ed5\u69d8<\/h3>\n
![\"\"](\"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-content\/uploads\/sites\/16\/2024\/01\/\u30e9\u30a4\u30c8\u30b7\u30fc\u30c8_\u63b2\u8f09\u5199\u771f-225x300.jpg\")
<\/p>\n
\n4x (NA 0.35, WD 16 mm)
\n12x (NA 0.53, WD 10.9 mm)<\/p>\n
\nCLARITY (RI 1.42-1.48)
\nDBE (RI 1.49-1.57)<\/p>\n
\n510\/20 (GFP narrow)
\n525\/50 (GFP wide)
\n545\/30 (YFP)
\n595\/40 (RFP)
\n650\/30 (mKate2, tdKatushka)
\n680\/30 (AF647, RedDot2)<\/p>\n\u4e88\u7d04\u8868\uff08Google Calendar\uff09<\/strong><\/h3>\n
\u64ae\u50cf\u306b\u3042\u305f\u3063\u3066\u306e\u6ce8\u610f\u70b9<\/strong><\/h3>\n
![\"\"](\"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn\/wp-content\/uploads\/sites\/16\/2024\/01\/Thy1-YFP-H-283x300.jpg\")
<\/p>\n\u30d7\u30ed\u30c8\u30b3\u30eb\u4f8b<\/h3>\n
\u30d5\u30ea\u30fc\u30bd\u30d5\u30c8\u3092\u4f7f\u3063\u305f\u89e3\u6790\u306e\u6d41\u308c<\/strong><\/h3>\n
\u8b1d\u8f9e\u306e\u8a18\u8f09\u65b9\u6cd5<\/strong><\/h3>\n
\u304a\u554f\u3044\u5408\u308f\u305b<\/strong><\/h3>\n