{"id":25,"date":"2017-05-19T14:00:42","date_gmt":"2017-05-19T05:00:42","guid":{"rendered":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/?page_id=25"},"modified":"2026-01-22T13:50:29","modified_gmt":"2026-01-22T04:50:29","slug":"resource","status":"publish","type":"page","link":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/resource\/","title":{"rendered":"Resources"},"content":{"rendered":"<h2>Resources<\/h2>\n<h3><b>SeeDB Resources<\/b><\/h3>\n<ul>\n<li><a href=\"https:\/\/sites.google.com\/site\/seedbresources\/\">SeeDB Resources<\/a>\u3000SeeDB, SeeDB2 and related information are summarized, including protocols, technical tips, and movies.<\/li>\n<\/ul>\n<p>Japanese version is\u00a0<a href=\"SeeDB-jp.pdf\">here<\/a>.<\/p>\n<p>SeeDB YouTube:\u00a0<a href=\"http:\/\/youtu.be\/pg2YOc5OcBI\">[YouTube(1)]<\/a>\u00a0<a href=\"http:\/\/youtu.be\/1tQ_ZiOFc60\">[YouTube(2)]<\/a> <a href=\"http:\/\/youtu.be\/1HNfghdQjko\">[YouTube(3)]<\/a><\/p>\n<p>SeeDB2 YouTube:\u00a0 <a href=\"https:\/\/youtu.be\/TakrFLY7pU4\">[YouTube(1)]<\/a> <a href=\"https:\/\/youtu.be\/ENz4NM_4OUI\">[YouTube(2)]<\/a><a href=\"https:\/\/youtu.be\/eSoCLNAMy8U\">[YouTube(3)]<\/a><\/p>\n<p>SeeDB and SeeDB2 are commercialzied from FUJIFILM Wako Pure Chemical: <a class=\"dhtgD\" href=\"http:\/\/www.google.com\/url?q=http%3A%2F%2Fwww.wako-chem.co.jp%2Fenglish%2Flabchem%2Fproduct%2Flife%2FSeeDB%2Findex.htm&amp;sa=D&amp;sntz=1&amp;usg=AFQjCNFzDFDkFABYEoAVonIEGrR_wqXuvw\" target=\"_blank\" rel=\"noopener noreferrer\">SeeDB Kit<\/a> and <a class=\"dhtgD\" href=\"http:\/\/www.google.com\/url?q=http%3A%2F%2Fwww.wako-chem.co.jp%2Fenglish%2Flabchem%2Fproduct%2Flife%2FSeeDB2%2Findex.htm&amp;sa=D&amp;sntz=1&amp;usg=AFQjCNHjQWGgha5i1nPF_o-J0YBQSEjYcg\" target=\"_blank\" rel=\"noopener noreferrer\">SeeDB2 Kit<\/a>.<\/p>\n<h3><b>NeuroMorph.org<\/b><\/h3>\n<p>Neuronal morphology data obtained with cleared brain tissues are deposited to NeuroMorph.Org. You can find our data through Browse &gt; By Archive &gt; Imai. <a href=\"http:\/\/neuromorpho.org\/bylab.jsp\">Here<\/a>\u00a0is the website (Ke et al., 2013; Ke et al., 2016; Murai et al., 2016).<\/p>\n<h3><strong>SSBD<\/strong>\u00a0(raw microscopy data)<\/h3>\n<p>Microscopy data from our lab are deposited to Systems Science of Biological Dynamics (SSBD) database. They are free to see, download, and re-use for research purposes.<\/p>\n<p><a href=\"http:\/\/ssbd.qbic.riken.jp\/image\/webclient\/?show=project-55\">Ke et al.,\u00a0<em>Nature Neuroscience<\/em>\u00a0(2013)<\/a><\/p>\n<p><a href=\"http:\/\/ssbd.qbic.riken.jp\/image\/webclient\/?show=project-59\">Ke et al.,\u00a0\u00a0<em>Cell Reports<\/em>\u00a0(2016)<\/a><\/p>\n<p><a href=\"http:\/\/ssbd.qbic.riken.jp\/image\/webclient\/?show=project-305\">Iwata et al.,\u00a0<em>Neuron<\/em>\u00a0(2017)<\/a><\/p>\n<p><a href=\"http:\/\/ssbd.qbic.riken.jp\/image\/webclient\/?show=project-401\">Sakaguchi et al.,\u00a0<em>eLife<\/em>\u00a0(2018)<\/a><\/p>\n<p><a href=\"http:\/\/ssbd.qbic.riken.jp\/set\/20200528\/\">Inagaki et al.,\u00a0<em>Cell Reports\u00a0<\/em>(2020)<\/a><\/p>\n<h3><strong>Addgene<\/strong>\u00a0(plasmids)<\/h3>\n<div style=\"width: 280px;border: thin solid #888;padding: 10px;text-align: center\"><a style=\"text-decoration: none;color: #585858;font-size: 16px;font-family: Arial,Helvetica,sans-serif\" href=\"https:\/\/www.addgene.org\/Takeshi_Imai\/\" target=\"_blank\" rel=\"noopener noreferrer\"><b>Takeshi Imai Lab<\/b><br \/>\nFind and request materials through<br \/>\n<img loading=\"lazy\" decoding=\"async\" style=\"margin-top: 16px\" src=\"https:\/\/media.addgene.org\/cms\/filer_public\/ea\/82\/ea826144-fce2-4c13-99e9-940af7e2853b\/logo-addgene.png\" alt=\"Addgene\" width=\"250\" height=\"48\" \/><\/a><\/div>\n<p>&nbsp;<br \/>\n<a href=\"https:\/\/www.addgene.org\/Takeshi_Imai\/\">Takeshi Imai Lab Plasmids<\/a><\/p>\n<h3><strong>GitHub<\/strong> (source code)<\/h3>\n<p><a href=\"https:\/\/github.com\/elifesciences-publications\/TetbowCodes\">Tetbow codes<\/a> from Sakaguchi et al., <em>eLife<\/em> (2018)<\/p>\n<p>&nbsp;<\/p>\n<h2>Protocols<\/h2>\n<h3><b>SeeDB, SeeDB2, Tetbow, &#8230;<\/b><\/h3>\n<ul>\n<li><a href=\"https:\/\/sites.google.com\/site\/seedbresources\/\">SeeDB Resources<\/a>\u3000SeeDB, SeeDB2 protocols and technical TIPs.<\/li>\n<li><a href=\"http:\/\/bio-protocol.org\/e1042\">SeeDB protocol in\u00a0<em>bio-protocol<\/em><\/a><\/li>\n<li><a href=\"https:\/\/bio-protocol.org\/e3046\">SeeDB2 protocol in\u00a0<em>bio-protocol<\/em><\/a><\/li>\n<li><a href=\"https:\/\/media.addgene.org\/cms\/filer_public\/d2\/98\/d298781e-9f55-469d-b092-caa15556513f\/how_to_use_tetbow.pdf\">Tetbow protocol in Addgene<\/a><\/li>\n<\/ul>\n<h3><b>Single-cell microarray<\/b> (Imai et al., 2009)<\/h3>\n<ul>\n<li><a href=\"single-cell-microarray.pdf\"> overview <\/a><\/li>\n<li><a href=\"TdTd0.txt\"> Single cell preparation <\/a><\/li>\n<li><a href=\"TdTd1.txt\"> T7 promoter-liked cDNA synthesis from single cells <\/a><\/li>\n<li><a href=\"TdTd2.txt\"> Cy Dye Labeling (for Agilent) <\/a><\/li>\n<li><a href=\"TdTd3.txt\"> Hybridization (Agilent 4x44k) <\/a><\/li>\n<li>*<a>&#8220;&gt;Quartz-Seq<\/a> is an updated version of single cell transcriptome analysis based on our original protocol.<\/li>\n<\/ul>\n<h3><b>Single-cell cDNA library <\/b> (Imai et al., 2006)<\/h3>\n<p><a href=\"single_cell_rtpcr.doc\"> Single cell cDNA library (simple version)<\/a><\/p>\n<h3><b>Two-color <i>in situ<\/i> hybridization<\/b> (Imai et al., 2006; Imai et al., 2009)<\/h3>\n<ul>\n<li><a href=\"ISH1.txt\"> Two-color <i>in situ<\/i> hybridization (TSA\/TSA) <\/a><\/li>\n<li><a href=\"ISH2.txt\"> Two-color <i>in situ<\/i> hybridization (TSA\/FastRed) <\/a><\/li>\n<li><a href=\"ISH3.txt\"> IHC\/ISH double staining<\/a><\/li>\n<\/ul>\n<h3><b>Other protocols<\/b><\/h3>\n<ul>\n<li><a href=\"bac.txt\"> BAC manipulation <\/a><\/li>\n<li><a href=\"ear.txt\">genotyping<\/a><\/li>\n<li><a href=\"ar.txt\">antigen retrieval<\/a><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h2>Mice<\/h2>\n<ul>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02931\"> I7-IRES-gapCFP <\/a><\/li>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02932\"> I7-IRES-Cre <\/a><\/li>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02933\"> I7(RDY)-IRES-gapYFP <\/a><\/li>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02934\"> caGs-IRES-gapYFP <\/a><\/li>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02935\"> I7(RDY)-IRES-caGs-IRES-gapCFP <\/a><\/li>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02936\"> I7-IRES-dnPKA-IRES-gapYFP <\/a><\/li>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02937\"> MOR23(del)-Venus <\/a><\/li>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02938\"> MOR23(del)-Cre-gapCFP line 17 <\/a><\/li>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02939\"> MOR23(del)-Cre-gapCFP line 6 (class II-Cre) <\/a><\/li>\n<li><a href=\"http:\/\/www.cdb.riken.jp\/arg\/TG_mutant_file\/CDB0449T.htmlt\"> I7(RDY)-IRES-Nrp1-IRES-gapCFP<\/a>\u00a0or\u00a0<a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC05279\">here<\/a><\/li>\n<li><a href=\"http:\/\/www2.brc.riken.jp\/lab\/animal\/detail.php?brc_no=RBRC02940\"> <i>Gng8<\/i> KO<\/a><\/li>\n<li>OMP-tTA BAC Tg (in prep)<\/li>\n<li>R26-TRE-GCaMP3 BAC Tg (in prep)<\/li>\n<\/ul>\n<p>Any questions and feedback are welcome!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Resources SeeDB Resources SeeDB Resources\u3000SeeDB, SeeDB2 and related information are summarized, including prot&hellip; <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/resource-template.php","meta":{"footnotes":""},"class_list":["post-25","page","type-page","status-publish","hentry","clearfix"],"_links":{"self":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/wp-json\/wp\/v2\/pages\/25","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/wp-json\/wp\/v2\/comments?post=25"}],"version-history":[{"count":20,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/wp-json\/wp\/v2\/pages\/25\/revisions"}],"predecessor-version":[{"id":516,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/wp-json\/wp\/v2\/pages\/25\/revisions\/516"}],"wp:attachment":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dn-en\/wp-json\/wp\/v2\/media?parent=25"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}