{"id":146,"date":"2013-08-02T13:21:19","date_gmt":"2013-08-02T04:21:19","guid":{"rendered":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/?page_id=146"},"modified":"2026-04-17T17:28:32","modified_gmt":"2026-04-17T08:28:32","slug":"research","status":"publish","type":"page","link":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/research\/","title":{"rendered":"\u7814\u7a76\u5185\u5bb9"},"content":{"rendered":"

\u30de\u30a6\u30b9\u521d\u671f\u767a\u751f\u306e\u6982\u7565<\/h3>\n

\u3000\u30de\u30a6\u30b9\u53d7\u7cbe\u5375\u306f\u3001\u5375\u5272\u3092\u7e70\u308a\u8fd4\u3057\u306a\u304c\u3089\u5375\u7ba1\u3092\u79fb\u52d5\u3057\u5b50\u5bae\u306b\u8fbf\u308a\u7740\u304d\u3001\u6804\u990a\u5916\u80da\u8449\uff08\u5c06\u6765\u306e\u80ce\u76e4\u3084\u80ce\u819c\u306b\u5bc4\u4e0e\uff09\u3068\u5185\u90e8\u7d30\u80de\u584a\uff08\u5c06\u6765\u306e\u4f53\u3092\u5f62\u6210\uff09\u304b\u3089\u306a\u308b\u80da\u76e4\u80de\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u5185\u90e8\u7d30\u80de\u584a\u306e\u5185\u8154\u5074\u3092\u539f\u59cb\u5185\u80da\u8449\u304c\u8986\u3044\u3001\u5185\u90e8\u7d30\u80de\u584a\u306f\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u3068\u547c\u3070\u308c\u308b\u7d44\u7e54\u306b\u306a\u308a\u307e\u3059\u3002\u80da\u306f\u7740\u5e8a\u3057\u305f\u5f8c\u3001\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u3068\u305d\u308c\u306b\u96a3\u63a5\u3057\u305f\u80da\u4f53\u5916\u5916\u80da\u8449\uff08\u6804\u990a\u5916\u80da\u8449\u7531\u6765\uff09\u304c\u5185\u8154\u5074\u3078\u3068\u5897\u6b96\u3057\u3066\u3044\u304d\u307e\u3059\u3002\u3084\u304c\u3066\u4e21\u8005\u306e\u5185\u90e8\u306b\u306f\u9023\u7d9a\u3057\u305f\u8154\u304c\u751f\u3058\u3001\u5468\u56f2\u3092\u81d3\u5074\u5185\u80da\u8449\u304c\u53d6\u308a\u56f2\u3093\u3060\u7b52\u72b6\u306e\u5375\u7b52\u80da\u3068\u547c\u3070\u308c\u308b\u72b6\u614b\u306b\u306a\u308a\u307e\u3059\uff08\u56f3\uff11\uff09\u3002<\/p>\n

\"\"
\n\u3010 \u56f3\uff11 \u3011<\/p>\n

\u3000\u54fa\u4e73\u985e\u3084\u9ce5\u985e\u306e\u4f53\u306f\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u304b\u3089\u4f5c\u3089\u308c\u307e\u3059\u304c\u3001\u3053\u306e\u5747\u4e00\u306a\u7d44\u7e54\u304b\u3089\u4f53\u304c\u4f5c\u3089\u308c\u308b\u305f\u3081\u306b\u306f\u524d\u5f8c\u3001\u80cc\u8179\u306e\u60c5\u5831\u304c\u5165\u529b\u3055\u308c\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u3001\u80da\u4f53\u5916\u5916\u80da\u8449\u3001\u81d3\u5074\u5185\u80da\u8449\u306e\u7d44\u7e54\u9593\u76f8\u4e92\u4f5c\u7528\u306b\u3088\u3063\u3066\u306a\u3055\u308c\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u80ce\u76e4\u3068\u53cd\u5bfe\u5074\u306e\u4f4d\u7f6e\u306b\u3042\u308b\u9060\u4f4d\u81d3\u5074\u5185\u80da\u8449\uff08DVE\uff09\u306e\u7d30\u80de\u7fa4\u306f\u3001\u5c06\u6765\u306e\u524d\u65b9\uff08\u982d\u5074\uff09\u306b\u5411\u304b\u3063\u3066\u79fb\u52d5\u3057\u524d\u65b9\u81d3\u5074\u5185\u80da\u8449\uff08AVE\uff09\u3092\u5f62\u6210\u3057\u307e\u3059\uff08\u56f3\uff11\uff09\u3002AVE\u306f\u3001Nodal\/Wnt\u306e\u963b\u5bb3\u56e0\u5b50\u3092\u5206\u6ccc\u3059\u308b\u3053\u3068\u3067\u524d\u65b9\u306e\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u306b\u304a\u3044\u3066\u3053\u308c\u3089\u306e\u30b7\u30b0\u30ca\u30eb\u3092\u6291\u5236\u3057\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u3001\u5f8c\u65b9\u306e\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u306b\u306f\u539f\u6761\u304c\u51fa\u73fe\u3057\u3001\u524d\u65b9\u306e\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u306f\u795e\u7d4c\u5916\u80da\u8449\u3068\u306a\u308a\u5c06\u6765\u306e\u8133\u3092\u5f62\u6210\u3057\u307e\u3059\u3002<\/p>\n

\u3000\u539f\u6761\u304b\u3089\u306f\u4e0a\u76ae\u9593\u8449\u8ee2\u63db\u306b\u3088\u3063\u3066\u4e2d\u80da\u8449\uff08\u521d\u671f\u306b\u306f\u5185\u80da\u8449\u3082\uff09\u304c\u751f\u3058\u3001\u5185\u80da\u8449\u3068\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u306e\u9593\u3092\u7e2b\u3063\u3066\u79fb\u52d5\u3057\u3001\u5375\u7b52\u80da\u306e\u3059\u3079\u3066\u306e\u9818\u57df\u306b\u5206\u5e03\u3059\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002\u539f\u6761\uff08\u5f8c\u306e\u5c3e\u82bd\uff09\u306e\u4e21\u5074\u306b\u306f\u4f53\u8ef8\u5e79\u7d30\u80de\u304c\u5b58\u5728\u3057\u3066\u304a\u308a\u3001\u795e\u7d4c\u5916\u80da\u8449\u53ca\u3073\u6cbf\u8ef8\u4e2d\u80da\u8449\u306b\u5206\u5316\u3059\u308b\u3053\u306e\u5e79\u7d30\u80de\u306f\u6301\u7d9a\u7684\u306b\u3053\u308c\u3089\u306e\u7d44\u7e54\u3092\u4f9b\u7d66\u3059\u308b\u3053\u3068\u3067\u3001\u80da\u306b\u306f\u4f53\u5e79\u90e8\u304c\u5f62\u6210\u3055\u308c\u4f38\u9577\u3057\u307e\u3059\uff08\u4f53\u8ef8\u4f38\u9577\uff09\u3002<\/p>\n

\u3000\u539f\u6761\u306e\u524d\u7aef\u306b\u306f\u30ce\u30fc\u30c9\u3068\u547c\u3070\u308c\u308b\uff12\u5c64\u6027\u306e\u69cb\u9020\u7269\u304c\u51fa\u73fe\u3057\u307e\u3059\u304c\u3001\u3053\u306e\u7d44\u7e54\u306f\u4f53\u7bc0\u3001\u795e\u7d4c\u7ba1\u3001\u8033\u80de\u306e\u30d1\u30bf\u30fc\u30cb\u30f3\u30b0\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3059\u810a\u7d22\u3092\u5f62\u6210\u3059\u308b\u3068\u5171\u306b\u3001\u30ce\u30fc\u30c9\u8179\u5074\u5c64\u306b\u306f\u5de6\u5411\u304d\u306e\u6c34\u6d41\u304c\u767a\u751f\u3057\u3001\u3053\u308c\u304c\u5de6\u53f3\u8ef8\u5f62\u6210\u306e\u5f15\u304d\u91d1\u306b\u306a\u308a\u307e\u3059\u3002\u6c34\u6d41\u306e\u60c5\u5831\u306f\u3001\u6700\u7d42\u7684\u306b\u5074\u677f\u4e2d\u80da\u8449\u306b\u304a\u3051\u308b\u5de6\u53f3\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u5dee\u7570\u306b\u8ee2\u63db\u3055\u308c\uff08\u56f3\uff12\uff09\u3001\u3053\u306e\u60c5\u5831\u306b\u57fa\u3065\u3044\u3066\u5404\u7a2e\u81d3\u5668\u306e\u539f\u57fa\u306f\u5de6\u53f3\u975e\u5bfe\u79f0\u306a\u5f62\u614b\u5f62\u6210\u3092\u884c\u3046\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

\"\"
\n\u3010 \u56f3\uff12 \u3011\u3000E8.2\u80da\u306b\u304a\u3051\u308bNodal\u3068Lefty1\u306e\u767a\u73fe<\/p>\n

\u3000\u30ce\u30fc\u30c9\u306e\u60c5\u5831\u304c\u5074\u677f\u4e2d\u80da\u8449\u306b\u3082\u305f\u3089\u3055\u308c\u3001\u5de6\u53f3\u975e\u5bfe\u79f0\u306a\u907a\u4f1d\u5b50\u767a\u73fe\u304c\u78ba\u7acb\u3055\u308c\u308b\u3002Nodal\uff08\u8d64\uff09\u3001Lefty1\uff08\u9752\uff09\u3002fp, \u795e\u7d4c\u5e95\u677f; lpm, \u5074\u677f\u4e2d\u80da\u8449\u3002\u80da\u9060\u4f4d\u5074\u304b\u3089\u89b3\u5bdf\u3002\u56db\u89d2\u306f\u30ce\u30fc\u30c9\u9818\u57df\u3092\u793a\u3057\u3066\u3044\u308b\u3002<\/p>\n

\u5de6\u53f3\u8ef8\u5f62\u6210<\/h3>\n

\u3000\u79c1\u305f\u3061\u306e\u4f53\u306f\u5916\u898b\u3053\u305d\u5de6\u53f3\u5bfe\u79f0\u3067\u3059\u304c\u3001\u5185\u81d3\u306f\u9855\u8457\u306a\u5de6\u53f3\u975e\u5bfe\u79f0\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u3059\u3079\u3066\u306e\u65b9\u304c\u540c\u3058\u975e\u5bfe\u79f0\u6027\u3092\u6301\u3064\u3053\u3068\u304b\u3089\u3001\u3053\u306e\u975e\u5bfe\u79f0\u6027\u306f\u767a\u751f\u5b66\u7684\u306b\u30d7\u30ed\u30b0\u30e9\u30e0\u3055\u308c\u305f\u3082\u306e\u3067\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002\u30d2\u30c8\u306b\u306f\u5185\u81d3\u9006\u4f4d\u7b49\u306e\u5de6\u53f3\u306b\u95a2\u9023\u3057\u305f\u5148\u5929\u7570\u5e38\u304c\u767a\u751f\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u3001\u591a\u304f\u306e\u5834\u5408\u3001\u5fc3\u81d3\u306b\u5947\u5f62\u3092\u4f34\u3044\u307e\u3059\u3002\u3053\u306e\u3053\u3068\u306f\u3001\u5de6\u53f3\u3092\u6b63\u3057\u304f\u6c7a\u5b9a\u3059\u308b\u3053\u3068\u306e\u5fc5\u8981\u6027\u3092\u793a\u3059\u3082\u306e\u3067\u3059\u304c\u3001\u679c\u305f\u3057\u3066\u3053\u308c\u3092\u53f8\u308b\u5de6\u53f3\u8ef8\u306f\u767a\u751f\u904e\u7a0b\u3067\u3069\u306e\u3088\u3046\u306b\u5f62\u6210\u3055\u308c\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n

\u3000\u79c1\u305f\u3061\u306f\u3001\u30de\u30a6\u30b9\u521d\u671f\u80da\uff08E8.2\uff09\u3067\u5de6\u53f3\u975e\u5bfe\u79f0\u306b\u767a\u73fe\u3059\u308b\u907a\u4f1d\u5b50Lefty1<\/em>\u3068Lefty2<\/em>\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u304c (Meno et al, Nature 1996, Meno et al, Genes to Cells 1997)\u3001\u3053\u306e\u767a\u898b\u304c\u7cf8\u53e3\u306b\u306a\u3063\u3066\u5de6\u53f3\u8ef8\u5f62\u6210\u306e\u89e3\u6790\u304c\u5927\u304d\u304f\u9032\u5c55\u3059\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3057\u305f\uff08\u5927\u962a\u5927\u5b66\u6ff1\u7530\u6559\u6388\uff08\u73fe\u3001National Center for Biological Sciences, India\uff09\u304c\u3001\u3053\u306e\u5206\u91ce\u3092\u727d\u5f15\u3057\u307e\u3057\u305f\uff09\u3002\u73fe\u5728\u3067\u306f\u3001\u5de6\u53f3\u8ef8\u5f62\u6210\u306f\u4ee5\u4e0b\u306e\u901a\u308a\u306b\u9032\u884c\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u3000\u30ce\u30fc\u30c9\u8179\u5074\u5c64\u306b\u5b58\u5728\u3059\u308b\u4e00\u6b21\u7e4a\u6bdb\u304c\u6642\u8a08\u56de\u308a\u306b\u56de\u8ee2\u3057\u3001\u5de6\u5411\u304d\u306e\u6c34\u6d41\uff08\u30ce\u30fc\u30c9\u6d41\uff09\u3092\u5f62\u6210\u3059\u308b\u3002\u3053\u306e\u6c34\u6d41\u306f\u3001\u30ce\u30fc\u30c9\u8fba\u7e01\u306ecrown cell\u306b\u304a\u3051\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u306b\u5f71\u97ff\u3057\u3001\u5e7e\u3064\u304b\u306e\u907a\u4f1d\u5b50\u304c\u5de6\u53f3\u975e\u5bfe\u79f0\u306b\u767a\u73fe\u3059\u308b\u3002\u6b21\u3044\u3067\u3001\u30ce\u30fc\u30c9\u8fd1\u508d\u306e\u5de6\u5074\u5074\u677f\u4e2d\u80da\u8449\u3067TGF\u03b2\u30d5\u30a1\u30df\u30ea\u30fc\u306eNodal<\/em>\u304c\u767a\u73fe\u3092\u958b\u59cb\u3057\u3001\u767a\u73fe\u57df\u304c\u524d\u5f8c\u8ef8\u306b\u6cbf\u3063\u3066\u5de6\u5074\u5074\u677f\u4e2d\u80da\u8449\u5168\u57df\u306b\u62e1\u5f35\u3059\u308b\u3002Nodal\u30b7\u30b0\u30ca\u30eb\u306f\u8ee2\u5199\u56e0\u5b50Pitx2\u306e\u767a\u73fe\u3092\u8a98\u5c0e\u3057\u3001\u5de6\u5074\u5074\u677f\u4e2d\u80da\u8449\u306e\u7d30\u80de\u7cfb\u8b5c\u3067Pitx2<\/em>\u767a\u73fe\u304c\u7dad\u6301\u3055\u308c\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u5de6\u53f3\u975e\u5bfe\u79f0\u306a\u5f62\u614b\u304c\u751f\u3058\u308b\u3001\u3068\u3044\u3046\u3082\u306e\u3067\u3059\u3002<\/p>\n

\u3000\u79c1\u305f\u3061\u306f\u3001\u5185\u81d3\u306e\u5de6\u53f3\u6027\u304c\u9006\u8ee2\u3059\u308b\uff08\u5b8c\u5168\u5185\u81d3\u9006\u4f4d\uff09\u5909\u7570\u30de\u30a6\u30b9inv<\/em>\u306e\u8868\u73fe\u578b\u767a\u73fe\u306e\u6a5f\u5e8f\u306e\u4e00\u7aef\u3092\u660e\u3089\u304b\u306b\u3057\uff08Oki et al., Development 2009\uff09\u3001\u30ce\u30fc\u30c9crown cell\u306b\u304a\u3051\u308bNodal\u3068\u305d\u306e\u963b\u5bb3\u56e0\u5b50Cerl2\u767a\u73fe\u306e\u975e\u5bfe\u79f0\u6027\u306e\u30d0\u30e9\u30f3\u30b9\u304c\u3001\u5074\u677f\u4e2d\u80da\u8449\u306b\u304a\u3051\u308bNodal\u767a\u73fe\u3092\u898f\u5b9a\u3059\u308b\u3068\u3044\u3046\u30e2\u30c7\u30eb\u3092\u63d0\u5531\u3057\u307e\u3057\u305f\u3002Nodal<\/em>\u306b\u306f\u3001\u81ea\u3089\u306e\u767a\u73fe\u3092\u8a98\u5c0e\u3059\u308b\u30dd\u30b8\u30c6\u30a3\u30d6\u30eb\u30fc\u30d7\u306e\u6a5f\u69cb\u304c\u3042\u308a\u307e\u3059\u3002Nodal\u306e\u6d3b\u6027\u306f\u30ce\u30fc\u30c9\u5de6\u5074\u304c\u5f37\u304f\u3001\u6050\u3089\u304f\u5de6\u5074\u5074\u677f\u4e2d\u80da\u8449\u306b\u5148\u306b\u62e1\u6563\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u305d\u3053\u3067Nodal<\/em>\u767a\u73fe\u3092\u8a98\u5c0e\u3057\u307e\u3059\u3002\u4e00\u5ea6\u5de6\u5074\u3067Nodal<\/em>\u767a\u73fe\u304c\u958b\u59cb\u3059\u308b\u3068\u3001\u305d\u306e\u30d5\u30a3\u30fc\u30c9\u30d0\u30c3\u30af\u963b\u5bb3\u56e0\u5b50\u3067\u3042\u308bLefty\u304c\uff08Meno et al., Molecular Cell 1999, Yamamoto et al., Development 2003\uff09\u3001\u53f3\u5074\u306b\u304a\u3051\u308bNodal<\/em>\u767a\u73fe\u3092\u6291\u5236\u3059\u308b\u3053\u3068\u3067\u4e00\u5074\u65b9\u6027\u306eNodal<\/em>\u767a\u73fe\u3092\u4fdd\u8a3c\u3057\u307e\u3059\uff08Nakamura et al., Developmental Cell 2006\uff09\u3002\u5c40\u6240\u7684\u306b\u59cb\u307e\u3063\u305f\u5de6\u5074\u306eNodal<\/em>\u767a\u73fe\u306f\u3001\u30dd\u30b8\u30c6\u30a3\u30d6\u30eb\u30fc\u30d7\u3067\u5de6\u5074\u5074\u677f\u4e2d\u80da\u8449\u5168\u57df\u306b\u62e1\u5f35\u3057\uff08Yamamoto et al., Development 2003\uff09\u3001\u524d\u5f8c\u8ef8\u306b\u6cbf\u3063\u3066\u5fc3\u81d3\u539f\u57fa\u53ca\u3073\u539f\u59cb\u8178\u7ba1\u306e\u5de6\u5074\u304c\u300c\u5de6\u5074\u5316\u300d\u3055\u308c\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u795e\u7d4c\u5e95\u677f\u53ca\u3073\u5de6\u5074\u5074\u677f\u4e2d\u80da\u8449\u3067\u767a\u73fe\u3059\u308bLefty\u306fNodal\u30b7\u30b0\u30ca\u30eb\u3092\u6291\u5236\u3059\u308b\u3053\u3068\u3067\u3001Nodal<\/em>\u304c\u53f3\u5074\u5074\u677f\u4e2d\u80da\u8449\u3067\u767a\u73fe\u3059\u308b\u3053\u3068\u3092\u6291\u5236\u3057\u3001\u3053\u308c\u306b\u3088\u3063\u3066Nodal<\/em>\u767a\u73fe\u57df\u304c\u78ba\u7acb\u3055\u308c\u307e\u3059\uff08Meno et al., Cell 1998, Meno et al, Developmental Cell 2001\uff09\u3002\u3053\u306eNodal-Lefty\u306e\u30b7\u30b9\u30c6\u30e0\u304c\u7834\u7dbb\u3059\u308b\u3068\u3001\u5185\u81d3\u9006\u4f4d\u3084\u5de6\u5074\u76f8\u540c\uff08\u53f3\u5074\u304c\u300c\u5de6\u5074\u5316\u300d\uff09\u3001\u53f3\u5074\u76f8\u540c\uff08\u5de6\u5074\u304c\u300c\u53f3\u5074\u5316\u300d\uff09\u304c\u751f\u3058\u3001\u3053\u308c\u306b\u4f34\u3063\u3066\u8907\u96d1\u5fc3\u5947\u5f62\u304c\u767a\u751f\u3057\u307e\u3059\u3002<\/p>\n

\u3000\u79c1\u305f\u3061\u306f\u3001Wnt3<\/em>\u304c\u30ce\u30fc\u30c9crown cell\u306b\u304a\u3044\u3066\u5de6\u53f3\u975e\u5bfe\u79f0\u306b\u767a\u73fe\u3059\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\uff08Kitajima et al., Developmental Biology 2013\uff09\u3002Wnt\u30b7\u30b0\u30ca\u30eb\u306fCerl2<\/em>\u767a\u73fe\u306e\u6291\u5236\u306b\u95a2\u308f\u308a\u3001Wnt3<\/em>\u306e\u767a\u73fe\u57df\u306f\u8ee2\u5199\u56e0\u5b50Foxa2\u3068Rbpj\u306e\u7d44\u5408\u308f\u305b\u3067\u898f\u5b9a\u3055\u308c\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002Rbjp\u306fNotch\u30b7\u30b0\u30ca\u30eb\u3092\u53d7\u3051\u308b\u3068\u3001\u305d\u306e\u6a5f\u80fd\u306f\u8ee2\u5199\u306e\u6291\u5236\u304b\u3089\u6d3b\u6027\u5316\u306b\u8ee2\u63db\u3057\u307e\u3059\u304c\u3001\u8208\u5473\u6df1\u3044\u3053\u3068\u306bNodal<\/em>\u3082\u540c\u3058\u4ed5\u7d44\u307f\u3067\u767a\u73fe\u57df\u304c\u898f\u5b9a\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u30ce\u30fc\u30c9crown cell\u3067\u767a\u73fe\u3059\u308b\u5de6\u53f3\u8ef8\u95a2\u9023\u907a\u4f1d\u5b50\u7fa4\u304c\u3001Notch\u30b7\u30b0\u30ca\u30eb\u306b\u3088\u3063\u3066\u30bb\u30c3\u30c8\u3068\u3057\u3066\u767a\u73fe\u8a98\u5c0e\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\u3002\u30ce\u30fc\u30c9\u306f\u3001\u6c34\u6d41\u3092\u767a\u751f\u3055\u305b\u308b\u3060\u3051\u3067\u306f\u306a\u304f\u3001Nodal\u30b7\u30b0\u30ca\u30eb\u306e\u5236\u5fa1\u306b\u5fc5\u8981\u306a\u5de6\u53f3\u8ef8\u95a2\u9023\u907a\u4f1d\u5b50\u3092\u540c\u8abf\u3057\u3066\u767a\u73fe\u3055\u305b\u3001\u305d\u308c\u3089\u306e\u5de6\u53f3\u975e\u5bfe\u79f0\u6027\u3092\u5236\u5fa1\u3059\u308b\u5de7\u307f\u306a\u5de6\u53f3\u8ef8\u88c5\u7f6e\u3068\u3057\u3066\u6a5f\u80fd\u3057\u3066\u3044\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u3000\u30ce\u30fc\u30c9\u306f\u767a\u751f\u904e\u7a0b\u306b\u65bc\u3051\u308b\u4e00\u904e\u7684\u306a\u69cb\u9020\u3067\u3059\u304c\u3001\u305d\u306e\u6a5f\u80fd\u767a\u73fe\u306f\u4ed6\u306e\u767a\u751f\u30a4\u30d9\u30f3\u30c8\u3068\u30ea\u30f3\u30af\u3057\u3066\u3044\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u539f\u6761\u9818\u57df\u306eFgf\u30b7\u30b0\u30ca\u30eb\u306f\u4e0a\u76ae\u9593\u8449\u8ee2\u63db\u306b\u3088\u3063\u3066\u4e2d\u80da\u8449\u3092\u751f\u307f\u51fa\u3059\u305f\u3081\u306b\u5fc5\u9808\u306e\u5f79\u5272\u3092\u62c5\u3044\u307e\u3059\u304c\u3001\u5408\u308f\u305b\u3066\u4e2d\u80da\u8449\u3067\u8ee2\u5199\u56e0\u5b50Tbx6\u306e\u767a\u73fe\u3092\u8a98\u5c0e\u3057\u307e\u3059\u3002Tbx6\u306f\u3001\u30ce\u30fc\u30c9\u3092\u53d6\u308a\u5dfb\u304f\u69d8\u306bNotch\u30ea\u30ac\u30f3\u30c9Dll1\u306e\u767a\u73fe\u3092\u8a98\u5c0e\u3057\u3001\u3053\u308c\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u30ce\u30fc\u30c9crown cell\u306b\u5165\u308b\u3053\u3068\u306b\u3088\u3063\u3066Nodal<\/em>\u767a\u73fe\u304c\u8a98\u5c0e\u3055\u308c\u307e\u3059\u3002\u5f93\u3063\u3066\u3001\u77ed\u6642\u9593\u306eFgf\u30b7\u30b0\u30ca\u30eb\u306e\u6291\u5236\u3067\u3082\u30ce\u30fc\u30c9\u306eNodal\u767a\u73fe\u306f\u6d88\u5931\u3057\u3001\u5de6\u5074\u5074\u677f\u4e2d\u80da\u8449\u306eNodal<\/em>\u767a\u73fe\u3082\u51fa\u73fe\u3057\u306a\u3044\u3053\u3068\u306b\u306a\u308a\u307e\u3059\uff08Oki et al., Developmental Dynamics 2010\uff09\u3002<\/p>\n

\u3000\u7cd6\u5c3f\u75c5\u306e\u5973\u6027\u304c\u598a\u5a20\u3059\u308b\u3068\u3001\u5b50\u4f9b\u306b\u69d8\u3005\u306a\u5148\u5929\u7570\u5e38\u304c\u767a\u751f\u3059\u308b\u30ea\u30b9\u30af\u304c\u4ea2\u9032\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u7cd6\u5c3f\u75c5\u306b\u3088\u308b\u5148\u5929\u7570\u5e38\u306b\u306f\u3001\u5148\u5929\u6027\u5fc3\u75be\u60a3\u3084\u81d3\u5668\u306e\u5de6\u53f3\u5f62\u614b\u7570\u5e38\u304c\u542b\u307e\u308c\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u7cd6\u5c3f\u75c5\u30de\u30a6\u30b9\u306e\u80da\u306b\u304a\u3051\u308b\u5de6\u53f3\u8ef8\u5f62\u6210\u3092\u89e3\u6790\u3057\u305f\u3068\u3053\u308d\u3001\u5de6\u5074\u5074\u677f\u4e2d\u80da\u8449\u306ePitx2<\/em>\u767a\u73fe\u304c\u6d88\u5931\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u3088\u3046\u306a\u72b6\u6cc1\u306f\u3001\u53f3\u5074\u76f8\u540c\u306e\u767a\u751f\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002\u9ad8\u6fc3\u5ea6\u30b0\u30eb\u30b3\u30fc\u30b9\u3092\u6dfb\u52a0\u3057\u305f\u5168\u80da\u57f9\u990a\u3067\u89e3\u6790\u3092\u3059\u308b\u3068\u3001\u9ad8\u30b0\u30eb\u30b3\u30fc\u30b9\u80da\u306e\u30ce\u30fc\u30c9\u3067\u306fNodal\u306e\u6d3b\u6027\u304c\u4f4e\u4e0b\u3057\u3066\u304a\u308a\u3001\u3053\u308c\u304c\u539f\u56e0\u3068\u306a\u3063\u3066\u5074\u677f\u4e2d\u80da\u8449\u306eNodal<\/em>–Pitx2<\/em>\u767a\u73fe\u304c\u6d88\u5931\u3057\u305f\u3068\u8003\u3048\u3089\u308c\u307e\u3057\u305f\u3002\u30ce\u30fc\u30c9\u306b\u304a\u3051\u308b\u5de6\u53f3\u8ef8\u95a2\u9023\u907a\u4f1d\u5b50\u7fa4\u306e\u767a\u73fe\u306f\u63c3\u3063\u3066\u6291\u5236\u3055\u308c\u3066\u304a\u308a\u3001\u3053\u308c\u3089\u3092\u8a98\u5c0e\u3059\u308bNotch\u30b7\u30b0\u30ca\u30eb\u306e\u7570\u5e38\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\uff08Hachisuga et al., PNAS 2015\uff09\u3002<\/p>\n

\u73fe\u5728\u3001\u5de6\u53f3\u8ef8\u5f62\u6210\u306e\u4ed5\u7d44\u307f\u3084\u95a2\u9023\u3057\u305f\u5148\u5929\u7570\u5e38\u306e\u89e3\u6790\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u524d\u5f8c\u8ef8\u5f62\u6210<\/h3>\n

\u3000Rosa Beddington\u3089\u306e\u30b0\u30eb\u30fc\u30d7\u306b\u3088\u3063\u3066\u3001\u30de\u30a6\u30b9\u5375\u7b52\u80da\u306e\u9060\u4f4d\u7aef\u306b\u3042\u308b\u9060\u4f4d\u81d3\u5074\u5185\u80da\u8449\uff08DVE\uff09\u306e\u7d30\u80de\u96c6\u56e3\u304c\u4e00\u5074\u65b9\u5411\u3078\u79fb\u52d5\u3057\u3001\u79fb\u52d5\u3057\u305f\u5074\u304c\u80da\u306e\u524d\u65b9\uff08\u982d\u5074\uff09\u306b\u306a\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u524d\u8ff0\u306e\u524d\u65b9\u81d3\u5074\u5185\u80da\u8449\uff08AVE\uff09\u304b\u3089\u306e\u4f5c\u7528\u306b\u3088\u3063\u3066\u80da\u306b\u306f\u524d\u5f8c\u8ef8\u304c\u78ba\u7acb\u3055\u308c\u307e\u3059\u3002\u79c1\u305f\u3061\u306f\u3001DVE\u304c\u79fb\u52d5\u3092\u958b\u59cb\u3059\u308b\u524d\u304b\u3089\u3001Nodal\u306e\u963b\u5bb3\u56e0\u5b50Lefty1\u3068Cerl\u304cDVE\u9818\u57df\u3067\u975e\u5bfe\u79f0\u306b\u50be\u3044\u3066\u767a\u73fe\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002DVE\u306f\u3053\u306e\u50be\u3044\u305f\u5074\u3078\u3068\u79fb\u52d5\u3057\u3001Lefty1<\/em>\u3068Cerl<\/em>\u3092\u7570\u6240\u7684\u306b\u5c40\u6240\u767a\u73fe\u3055\u305b\u308b\u3068\u3001\u767a\u73fe\u3057\u305f\u65b9\u5411\u3078DVE\u304c\u79fb\u52d5\u3059\u308b\u3053\u3068\u304b\u3089\u3001DVE\u9818\u57df\u306b\u304a\u3051\u308b\u3053\u308c\u3089\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u975e\u5bfe\u79f0\u6027\u306fDVE\u306e\u79fb\u52d5\u65b9\u5411\u3068\u79fb\u52d5\u305d\u306e\u3082\u306e\u3092\u898f\u5b9a\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\uff08Yamamoto et al., Nature 2004\uff09\u3002AVE\u306e\u4f5c\u7528\u306b\u3088\u3063\u3066\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u306b\u306f\u524d\u5f8c\u306e\u9055\u3044\u304c\u751f\u3058\u3001\u5f8c\u65b9\u306b\u539f\u6761\u304c\u5f62\u6210\u3055\u308c\u307e\u3059\u3002\u539f\u6761\u304b\u3089\u306f\u4e2d\u80da\u8449\u3068\u521d\u671f\u306b\u306f\u5185\u80da\u8449\u304c\u51fa\u73fe\u3057\u3001\u80da\u5168\u4f53\u306b\u884c\u304d\u6e21\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u539f\u6761\u306e\u4e21\u5074\u65b9\u306b\u306f\u4f53\u8ef8\u5e79\u7d30\u80de\u304c\u5b58\u5728\u3057\u3066\u304a\u308a\u3001\u795e\u7d4c\u4e0a\u76ae\u7d30\u80de\u3068\u6cbf\u8ef8\u4e2d\u80da\u8449\u3092\u7d99\u7d9a\u3057\u3066\u4f9b\u7d66\u3059\u308b\u3053\u3068\u3067\u4f53\u5e79\u90e8\u3092\u751f\u307f\u51fa\u3057\u307e\u3059\u3002\u79c1\u305f\u3061\u306f\u3001\u4f53\u306e\u3059\u3079\u3066\u306e\u7d44\u7e54\u3092\u4f5c\u308b\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u306e\u591a\u5206\u5316\u80fd\u306e\u5909\u9077\u306b\u8208\u5473\u3092\u6301\u3061\u3001\u69d8\u3005\u306a\u30a2\u30d7\u30ed\u30fc\u30c1\u306b\u3088\u3063\u3066\u3053\u308c\u3089\u306e\u767a\u751f\u73fe\u8c61\u306e\u89e3\u660e\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3059\u3002<\/p>\n

\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u5e79\u7d30\u80de<\/h3>\n

\u3000\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u5e79\u7d30\u80de\uff08EpiSC\uff09\u306f\u3001\u30de\u30a6\u30b9\u80da\u306e\u30a8\u30d4\u30d6\u30e9\u30b9\u30c8\u3092\u5358\u96e2\u57f9\u990a\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u6a39\u7acb\u3055\u308c\u308b\u5e79\u7d30\u80de\u3067\u3059\u3002\u30de\u30a6\u30b9\u3084\u30d2\u30c8\u306e\u80da\u76e4\u80de\u304b\u3089\u6a39\u7acb\u3055\u308c\u308b\u306e\u304c\u80da\u6027\u5e79\u7d30\u80de\uff08mESC, hESC\uff09\u3067\u3059\u304c\u3001EpiSC\u306fhESC\u306b\u6027\u8cea\u304c\u8fd1\u3044\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u307e\u3057\u305f\u3002\u73fe\u5728\u3067\u306f\u3001mESC\u3092\u30ca\u30a4\u30fc\u30d6\u578b\u3001EpiSC\u3084hESC\u3001\u30d2\u30c8iPS\u7d30\u80de\u306f\u30d7\u30e9\u30a4\u30e0\u578b\u306b\u5206\u985e\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30e0\u578b\u591a\u80fd\u6027\u5e79\u7d30\u80de\u306e\u7279\u5fb4\u306e\u4e00\u3064\u3068\u3057\u3066\u3001\u7d30\u80de\u306e\u7dad\u6301\u3084\u6a39\u7acb\u304c\u96e3\u3057\u3044\u3053\u3068\u304c\u4e0a\u3052\u3089\u308c\u307e\u3059\u3002\u79c1\u305f\u3061\u306f\u3001\u30de\u30a6\u30b9\u521d\u671f\u767a\u751f\u306e\u5206\u5b50\u6a5f\u69cb\u306e\u77e5\u898b\u3092\u5fdc\u7528\u3059\u308b\u3053\u3068\u3067\u3001EpiSC\u306e\u57f9\u990a\u53ca\u3073\u6a39\u7acb\u52b9\u7387\u3092\u5287\u7684\u306b\u6539\u5584\u3059\u308b\u57f9\u990a\u65b9\u6cd5\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\uff08Sumi et al., 2013\uff09\u3002<\/p>\n

\u5185\u8033\u5f62\u6210<\/h3>\n

\u3000\u5185\u8033\u306e\u819c\u8ff7\u8def\u306f\u8907\u96d1\u306a\u5f62\u72b6\u3092\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u306e\u5f62\u614b\u3092\u751f\u307f\u51fa\u3057\u69cb\u6210\u7d30\u80de\u3092\u7684\u78ba\u306b\u5206\u5316\u30fb\u914d\u7f6e\u3055\u305b\u308b\u3053\u3068\u306f\u3001\u5185\u8033\u304c\u8074\u899a\u30fb\u5e73\u884c\u611f\u899a\u3092\u53f8\u308b\u5668\u5b98\u3068\u3057\u3066\u6a5f\u80fd\u3059\u308b\u305f\u3081\u306b\u5fc5\u9808\u3067\u3059\u3002\u5185\u8033\u819c\u8ff7\u8def\u306f\u3001\u767a\u751f\u904e\u7a0b\u3067\u5185\u8033\u30d7\u30e9\u30b3\u30fc\u30c9\u3068\u547c\u3070\u308c\u308b\u4e0a\u76ae\u304c\u9665\u5165\u3057\u3066\u751f\u3058\u305f\u5358\u7d14\u306a\u4e0a\u76ae\u306e\u888b\u300c\u8033\u80de\u300d\u304b\u3089\u4f5c\u3089\u308c\u307e\u3059\u3002Wnt\u30ab\u30ce\u30cb\u30ab\u30eb\u30b7\u30b0\u30ca\u30eb\u306f\u8033\u80de\u306e\u300c\u80cc\u5074\u5316\u300d\u306b\u95a2\u308f\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3057\u305f\u304c\u3001\u5185\u8033\u767a\u751f\u5f8c\u671f\u306b\u304a\u3051\u308b\u5f79\u5272\u306f\u4e0d\u660e\u3067\u3057\u305f\u3002\u79c1\u305f\u3061\u306f\u3001Wnt\u30b7\u30b0\u30ca\u30eb\u304c\u80cc\u5074\u9818\u57df\u306b\u304a\u3044\u3066\u3082\u5916\u5074\u304b\u3089\u6d88\u5931\u3057\u3066\u884c\u304f\u3053\u3068\u3092\u898b\u51fa\u3057\u3001\u3053\u306e\u30b7\u30b0\u30ca\u30eb\u306e\u6291\u5236\u304c\u534a\u898f\u7ba1\u5f62\u6210\u306b\u5fc5\u8981\u3067\u3042\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\uff08Noda et al., 2012\uff09\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u30de\u30a6\u30b9\u521d\u671f\u767a\u751f\u306e\u6982\u7565 \u3000\u30de\u30a6\u30b9\u53d7\u7cbe\u5375\u306f\u3001\u5375\u5272\u3092\u7e70\u308a\u8fd4\u3057\u306a\u304c\u3089\u5375\u7ba1\u3092\u79fb\u52d5\u3057\u5b50\u5bae\u306b\u8fbf\u308a\u7740\u304d\u3001\u6804\u990a\u5916\u80da\u8449\uff08\u5c06\u6765\u306e\u80ce\u76e4\u3084\u80ce\u819c\u306b\u5bc4\u4e0e\uff09\u3068\u5185\u90e8\u7d30\u80de\u584a\uff08\u5c06\u6765\u306e\u4f53\u3092\u5f62\u6210\uff09\u304b\u3089\u306a\u308b\u80da\u76e4\u80de\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u5185\u90e8\u7d30\u80de\u584a\u306e\u5185\u8154\u5074\u3092\u539f\u59cb\u5185\u80da\u8449\u304c\u8986\u3044\u3001…\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":5,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-146","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/wp-json\/wp\/v2\/pages\/146","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/wp-json\/wp\/v2\/comments?post=146"}],"version-history":[{"count":18,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/wp-json\/wp\/v2\/pages\/146\/revisions"}],"predecessor-version":[{"id":555,"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/wp-json\/wp\/v2\/pages\/146\/revisions\/555"}],"wp:attachment":[{"href":"https:\/\/www.lab.med.kyushu-u.ac.jp\/dev\/wp-json\/wp\/v2\/media?parent=146"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}