第３５回生命医科学セミナー （Biomedical Science Seminar

演 題：

An epithelial program driven by KLF4 stoichiometry determines hallmarks of somatic cell reprogramming

演 者：　 Dr. Knut Woltjen

Dept. of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University

日 時：平成28年2月16日（火）

１７：３０～１９：００

場 所：病院地区総合研究棟１階　１０２講義室

Through the expression of Oct3/4, Sox2, Klf4, and c-Myc, somatic cells reprogram to induced pluripotent stem cells (iPSCs), yet the exact mechanisms remain to be elucidated. We employed a piggyBac transposon vector system to assess the cellular and molecular hallmarks of mouse somatic cell reprogramming, and revealed a KLF4 cDNA variation that ultimately affects KLF4 protein levels. As a result, high-KLF4 generates few, but fully pluripotent iPSCs. In contrast, low-KLF4 gives rise to mostly partially reprogrammed cells.

Using expression analysis at intermediate reprogramming stages, we observed a dominant epithelial gene expression profile in the high-KLF4 condition. We identified cell surface markers and confirmed their presentation by FACS. Using CRISPR/Cas9, we performed a focused screen for modulators of the mesenchymal-to-epithelial transition (MET) by gene knockout. We are now employing these markers to fractionate cells with the intention of identifying intermediate cell populations that contribute to “true” iPSCs. Beyond reprogramming, I will discuss how our findings have initiated an active branch of research into human epidermal development and genodermatoses.

多くの方の参加を歓迎致します。

共　催：福岡医学会

問合せ：九州大学大学院 医学研究院 基礎放射線医学分野　續　輝久（内線6141）

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